Comparing Body Weight, Landing Hindlimb Footsplay and Serum Testostron Concentration Following Chronic Low Dose Acrylamide Administration in Male Rats

Acrylamide monomer causes peripheral neurotoxicity, mutagenicity, clastogenicity, male reproductive toxicity, prenatal lethality, and endocrine-related tumors in rodents. The main purpose of this study was to determine the toxicopathologic effects of chronic low doses of ACR on the male rat serum testosterone concentration and its relation to neurological indices including body weight gain and LHF. For this purpose, 40 male rats (Wistar, approximately 250 g) were selected. Rats were housed in polycarbonate boxes as 5 per each. Randomly assigned groups of rats (10 rats per exposure group. total 3 exposure groups as A, B and C) were exposed to 0.1, 1, 10mg/kg per day×5 days/week × 12 weeks p.o. (gavage) respectively. The remaining 10 rats were housed in group (D) as control group. Control rats received daily i.p. injections of 0.9% saline (3ml/kg). As indices of developing neurotoxicity, weight gain and landing hind limb foot splay (LHF) were determined. Weight gains were measured daily prior to injection. The lowest percentage of body weight gain and the highest value of LHF were observed in rats belong to group C, which received the highest dose of ACR. In addition, serum testosterone concentration in Comparing with rats in control group D, significant decrease in serum testosterone concentration were observed in treatment groups and the lowest value in group C.Our results suggest that ACR adversely affect on growth, development and reproduction (serum testosterone concentration) of male rats.