Effects of singular or combined administration of prebiotioc AMax© and Lactobacillus casei on growth related genes expression (IGFI and GH) in common carp (Cyprinus carpio)

The aim of the present study was to investigate the effects of prebiotic AMAX, probiotic Lactobacillus casei and combined AMAX and Lactobacillus casei on growth hormone (GH) and Insulin like Growth Factor (IGF1) genes expression in common carp. For this purpose, 180 fish with average weight of 23.7+0.35 gr fed on diet supplemented with 0.1 % prebiotic, CFU/g Lactobacillus casei , the mix of AMAX and Lactobacillus casei  for 8 weeks. At the end of feeding trial, RNA extracted from liver and brain, cDNA synthesised using SuprimeScript RTase and growth genes expression were assessed using Real Time PCR. Result showed that expression of IGF1and GH was (5.94, 5.68 and 7.87) and (9.25, 8.8 and 17.35) fold of control group. There was significant differences in both studied gene in fish fed combined pro and pre with singular AMAX and Lactobacillus casei (p<0.05). Overall result showed suitable effect of prebiotic AMAX and probiotic Lactobacillus casei specially their combination on growth indices in common carp.