Methicillin and Mupirocin resistance genes in Staphylococcus aureus isolated from hospital staff in Darab by using of Multiplex

Methicillin and Mupirocin –resistance Staphylococcus aureus (MRSA) are considered as the important hospital pathogens. One of the important resources of this organism in nosocomial infection is related to hospital personnel. The objective of this research was is to investigate the frequency of MRSA gene in staphylococcus aureus isolated from Hospital staff in Darab by multiplex PCR.

In this study, 150 hospital staffs were investigated and specimens of staphylococcus aureus which isolated from those were confirmed by biochemical and microbial methods. The disk diffusion method was used to determine the susceptibility of the isolates to methicillin and mupirocin antibiotic. Then, the presence of mec-A, mup and nuc genes were investigated by using of Multiplex PCR method.

Among of 150 subjects investigated, 120 ones (80%) were female. Totally, 53 people (35.3%) were carriers of Staphylococcus aureus which 42 and 11 were female and male, respectively. Among of the 11 staphylococcus aureus isolated in men, 4 cases were methicillin-resistant and any of them were not mupirocin-resistant. Of the 42 staphylococcus aureus isolated in women studied, 11 cases were methicillin-resistant and 2 cases were mupirocin-resistant. Using of molecular method, 11 isolates (9.5%) were contained mec-A gene and any of isolates were not contained mup gen.

The frequency of methicillin- and mupirocin-resistant staphylococcus aureus among the personnel of Darab Hospital was relatively low. However, permanent control of the carriers and treatment of them can prevent the spread of this bacterium and its associated infections