Preparation of biological scaffolds derived from bladder sheep and evaluation of Bio compatibility and mechanical properties of the scaffold

This study was aimed to preparation of sheep urinary bladder derived from biological scaffold by a combined (physical and chemical) method and evaluation of scaffold biocompatibility.  

Urinary bladder decellularization was performed by physical and chemical methods. In the physical method, the bladder fragments were incubated at -4 °C for 24 h and intervaled the fragments each 6 h by placing for 10 min in 0.1% sodium azide solution. After 24 h, the samples were placed at -20 and -40 °C for 2 and 1 h, respectively. The bladder fragments were held in a cryotube and emerged in liquid nitrogen by five two-minute steps, and finally washed by the PBS buffer containing 0.1% sodium azide. In the chemical decellularization, all of the physically treated bladder fragments were placed in a sodium dodecyl sulphate solution under slow stirring for 24 h. The samples were rinsed with sterile distilled water, sterilized with 75% ethanol and 0.2% peracetic acid and finally were placed in PBS for 24 h.

The light and electron microscopey studies revealed the biocompatibility of seeded stem cells on the sheep bladder bioscaffold, in 3rd, 5th and 7th days. The most biocompatibility was observed in the end of 7th day.

Decellularized urinary bladder scaffold revealed biocompatibility which could be considered as a potential nontoxic and biocompatible bioscaffold for application in tissue engineering regenerative medicine.