Genetic Analysis NS Gene of H9N2 Subtype of Influenza Virus A Isolated in Iranian poultry industry in during 2007-2016

The H9N2 influenza virus is of great importance due to the wide range of hosts due to gene rearrangement, which facilitates the possibility across of the Inter-species barrier. This study demonstrates useful information on the homogeneity of the H9N2 strain of avian influenza virus in Iran with other influenza viruses in Pakistani and Israeli birds for years to come, which emphasizes the need for continuous surveillance of the influenza virus genes and the evolution of strains of the strains in the region. The present study was conducted on the 10 isolates NS gene sequence of the H9N2 influenza virus strain from 2007-2016. After amplification by RT-PCR and genome extraction, the genes were completely Sequencing (it was carried out with the same primer used for gene amplification) and then phylogenetic analysis. All of these 10 isolates were included in allele A under the Korean lineage with amino acid motif KSEI in the PDZ ligand, which shows the similarity of Pakistani virus genes to C of Iran. H9N2 viruses are not highly pathogenic, But by transferring to mammalian species like humans, they cause mild illness .These viruses, in addition to connecting to poultry receptors, have the ability to connect to receptors similar to human type strains with changes in amino acids.