Study of the effect of Lactobacillus buchneri supplementation to dairy diet contaminated with Aflatoxin B1 on in vitro gas production parameters

Aflatoxin B1 is produced by Aspergillus falvus and Aspergillus parasiticus which affect rumen motility and feed degradation. The aim of this study was to evaluate the effects of aflatoxin B1 on rumen fermentation in the presence of Lactobacillus buchneri additive using in vitro gas production technique. Experimental treatments were: basal diet, basal diet with 0.5 mg/ml aflatoxin B1, basal diet with 1 mg/ml aflatoxin B1, and aflatoxin B1 contaminated base diet (1 mg/ml) with addition levels of Lactobacillus buchneri 0.01, 0.1 and 0.2 ng/kg diet, respectively. Gas volume of each bottles were recorded at 2, 4, 6, 8, 12, 24, 36, 48, 72, 96 and 120 hours of incubation. The data were analyzed in a completely randomized design with three replications. According to the results of gas production, aflatoxin B1 affect the rumen fermentation and reduced gas production volume (429 ml) compared to control group (382 ml) (P <0.05). At 120 h incubation time, the highest and lowest gas production volume was related to control group  and treatment with 0.2 ng L. buchneri (P <0.05). Addition of Lactobacillus buchneri caused an increase in the estimated parameters include ME, NEL and organic matter digestibility. Also, the L. buchneri addition at levels of 0.01, 0.1 and 0.2 ng/kg significantly reduced pH of diet contaminated with aflatoxin B1 (P<0.05). According to the results it concluded that, in vitro digestibility increased by L. buchneri and it has ability to inhibit aflatoxin B1 reverse effect in in vitro rumen fermentation of dairy cattle diet.