Comparative study of the proliferation potency of adipose tissue derived stem cells from infrapatellar area, subcutaneous adipose tissue and tail fat pad in a sheep animal model

The stem cells are obtained from different sources and due to the role of them in the treatment and recovery of damaged tissues, characterizing and identifying of the sources of stem cells and investigating them is particularly interesting for researchers. Thus, the purpose of this study was to identify ideal tissue sources and capture stem cells with appropriate differentiation and proliferation potential.

The adipose tissue sample were harvested from the tail adipose tissue, subcutaneous and infrapatellar in the slaughterhouse from male sheep about 2 years old and were rinsed to small pieces in the laboratory and were treated by the collagenase enzyme and the cells were counted by Neubauer chamber with inverted microscope and then about 105 cells were cultured in T25 flask and was calculated the doubling time by deleted the Peterson formula Td=Tlg2/lg(Nt/N0). Data were analyzed using analysis of variance (P< 0/02).

Our findings showed that the stem cells of the three areas in the early passages are similar to each other in regarding proliferation and morphological potentials, but in higher passages as compared to the subcutaneous and tail fat, the cells of the infrapatellar region received confluence 80% in shorter time than the others.

The results of this study showed that infrapatellar adipose tissue stem cells maintain their proliferation potential and differentiation for long time as compared to adipose tissue of other areas. So infrapatellar fat pad can be an ideal source for obtaining stem cells.