Investigation on cytotoxic effect of hydroalcoholic extract of Artemisia sieberi on SKBr3cell line

This study was performed to evaluate the cytotoxic effects of hydroalcoholic extract of Artemisia sieberi and its effect on the cell cycle in the SKBr3 breast cancer cell line.

In this study, hydroalcoholic extract of Artemisia was prepared. SKBr3 cells were exposed to different concentrations (1, 10, 100, 1000 µgml-1) of extract at two different time intervals of 24 and 48 h. We employed MTT assay and flow cytometry analysis to evaluate effects of the prepared extract on the cell cycle characteristics and its cytotoxic properties.

Based on the data obtained from the MTT test, the highest toxicity of the extract observed at the concentration of 1000 µgml-1 within 48 h after the extract exposure. The IC50 of hydroalcoholic extract was 150 and 50 µgml-1 at 24 and 48 h, respectively. According to the data obtained from flow cytometry analysis, the extract arrests cell cycle in 24 and 48 h treatment groups.

The hydroalcoholic extract of Artemisia at certain concentrations inhibits the growth of SKBr3 breast cancer cells by ceasing cell cycle step.