Isolation and identification of Mycoplasmas which cause contagious Aga-lactia from sheep & goats in Tehran province

Agalactia, has been known as a contagious disease in Mediterranean countries, Africa, Europe, and west Asia e.g. Iran where the livestock were reared massively for approximately several years ago and it has been led to noticeable economic damages and losses. This disease appears by symptoms such as fever, inappetence, lameness and loss of milk in dairy ewes, reduction to total lack of lactation and abortion in pregnant livestock. No study has been so far conducted on isolation of Agalactia disease factor and determining its molecular identity in ovine and caprine herds in Tehran province. The current research aimed to isolate and determine molecular identity of Agalactia disease factor (Agalactiae mycoplasma) in sheep and goat that suffered from Agalactia, using culture and PCR methods in Tehran province. Among the total 33 taken samples, 14 samples were confirmed as mycoplasma species that showed specific band (bp163) on agarose gel and in one of these 14 given samples, the culture was positive.