Effect of iron nutrient on growth, physiological and biochemical trait of Echinaceae purpurea to water deficit treatments

In order to study the effects of irrigation period and iron nano-chelate on the some morphophysiological and biochemical characteristics of Echinaceae purpurea L., a experiment was conducted as factorial based on randomized complete block design with three replications at research farm of Baharan University of Gorgan, during growing season of 2017-2018. The treatments were four levels of irrigation periods (every 3 as control, 6, 9 days and 12 days) and iron nano-chelate (INC) including four levels (0 as control, 3, 6 and 9 in liter). Results showed that the irrigation periods increasing was decreased plant height, shoot wet and dry weight, root wet and dry weight. Highest irrigation period reduced in RWC and ion linkage. The antioxidant activity and soluble sugar were gone up under irrigation period and the maximum amount was in third level of irrigation. INC has significant effect on all traits except of shoot total phenol and shoot wet weight that the most effect was in third and fourth. Interaction effect of these treatments was showed that every 6 days and 6 in liter of INC increased soluble sugar and antioxidant activity and the most amount of proline was produced in every 12 days and 9 in liter of INC. Results of this study showed that drought stress increasing decreased morphological charactristics and RWC. However, proline, soluble sugar, total phenol, ion linkage and antioxidant activity were climbed. However, INC protects E. purpurea from drought stress and reduces damage caused by drought stress. Generally, 6 in liter of INC was advised for reducing of negative effects of irrigation regimes.