Considering of damage to the choroid plexus and increasing expression of AQP-1 canals after induction of cholestatic model in male Wistar rat.

Cholestasis is a model for creating Cirrhosis of the liver. The flux of water in the brain through membrane-anchored water channels, known as aquaporins (AQPs). Aquaporin-1 is a member of the family. there is in the central nervous system, it is possible that these proteins contribute to water transport across the blood-brain barrier. Mechanism of regulation of expression AQP1 is a complex and disruption of it can lead to cerebral edema, hydrocephalus, and other brain problems. The purpose of this study is evaluating the AQP-1 in cholestatic Wistar male rats’ choroid plexus and considering of choroid plexus tissue compared with the other groups.

This study was performed via bilateral bile duct ligation in rats. The animals were divided in to three groups, Control,Sham, with surgery but without bile duct ligation and cholestasis with surgery and cut the bile duct. After two weeks rats were sacrificed. Brain tissue and the amount of AQP-1 were determined by immunohistochemistry method and H & E staining.

In the central nervous system, AQP-1 was expressed only in the epithelium of the lateral ventricle’s choroid plexus. Rats with cholestasis showed increased AQP-1 protein in choroid plexus, also in histological observation by H & E staining, choroid plexus tissue damage in cholestatic samples was observed.

This study was conducted to investigate changes in the amount of AQP-1 protein. Also, It shows increased levels of AQP-1 in the choroid plexus of cholestatic rats which may cause brain damage and edema have been observed in cholestasis.