Effect of resistance training and Resveratrol supplementation on muscle regeneration of MyoD and eMHC in CT-26 colon cancer mice

Physical exercise and nutrition supplements are recommended interventions to reduce cachexia. This study was conducted to determine the effect of resistance training and resveratrol supplementation on muscle regeneration indices of MyoD (myoblast determination protein) and eMHC (embryonic Myosin Heavy Chain) in CT-26 colon cancer mice.

This experimental study was performed on 20 six-week-old BALB/c mice to which CT-26 tumor was implanted. The mice were divided into four groups of resistance training, resveratrol, combination of resveratrol with resistance training, and control. The resistance training group performed six weeks of progressive resistance training. The resveratrol group received 100 mg/kg resveratrol per day, and the control and resistance training group received the solution of Methyl cellulose through gavage. In gastrocnemius muscle MyoD protein and eMHC level were measured using western blot and ELISA methods, respectively.

eMHC protein in combination of resveratrol with exercise group (4.66±0.25) increased significantly compared to the exercise group (3.46±0.64) (P<0.05). Body weight of mice without tumor (21.50±1.30) in the resistance training group increased significantly compared to other groups (P<0.05). There was no significant difference in the tumor weight of mice and MyoD protein in expermental groups.

Regarding the increasing effect of combination of resveratrol with exercise group compared to resistance training group on eMHC, resveratrol supplementation at higher doses may be recommended along with resistance training to improve muscle regeneration.