Given the role of silver nanoparticles on oxidative stress and antioxidant royal jelly, this research aimed to study the simultaneous treatment of silver nanoparticles and royal jelly in the NMRI mice testis tissue.

In this experimental study, thirty adult male mice (NMRI) were used with an average weight (36±2) at the 5 groups (n=6): Control, treatment with silver nanoparticles (500 mg/kg/day), treatment with royal jelly (300 mg/kg/day) and simultaneous treatment silver nanoparticles +royal jelly (the offered doses), by orally administration for 35 days. After treatment, the total volume of testis, and its diameter and height of the germinal epithelium, total number spermatids, spermatocytes, Sertoli cells with stereological method were estimated. The total antioxidant capacity (TAC) by FRAP method), levels of malondialdehyde (MDA) by spectrophotometric method, and serum testosterone by Elisa kit were measured.

The total volume of testis, diameter and height of the germinal epithelium, total number spermatids, spermatocytes, Sertoli cells significantly decreased in silver nanoparticles group compared to the control group (P<0.001). A significant increase in level of MDA and significant decrease in total antioxidant capacity and serum testosterone levels was found in silver nanoparticles group compared with control group (P<0.001). Above parameters were significantly increased in silver nanoparticles +royal jelly group compared with silver nanoparticles group.

According to the results, royal jelly may be the effective in the protection against the adverse effects of silver nanoparticles and improve the function of male reproductive system.