Determination of aflatoxin B1 in broiler feed in thepoultry farms of isfahan province

Mycotoxins in animal feed transfer to animal products and thus cause some problem like cancer in human. Contaminated feed must be omitted for prevention of toxin transfer. This study was designed for determination of aflatoxin B1 levels in poultry diet. A total of 177 samples were collected randomly from broiler feeds from different farms and tested by ELISA. Results showed %98.9 contamination of total sample. Levels of aflatoxin B1 in positive samples ranged from 0.64 to 88.5µg/kg and the average contamination level of samples was estimated at 9.91 µg/kg. In the levels higher than 10µg/kg (permitted for broiler diet) the highest rate belonged to complete feed (46.2%) and soybean meal (33.4%). The results indicated that samples take in summer had more contamination. The difference between this season with others was signifcant (p<0.05). High temperature in summer causes more fungi reproduction and hence more toxin production. The difference between corn, wheat, soybean, barley and complete diet samples was not signifcant (p>0.05).