Effect of green algae extract; Spirogyra sp. on the rate of pectinase enzyme secretion and expression level of PgaA gene in Aspergillus niger

Aspergillusniger is one of the microorganisms that produce the pectinase enzymes group of the at high levels. In this fungus, PgaA genes code Glycoside hydrolase family 28 that secrete polygalacturonase enzymes. Green algae (Chlorophyceae) and blue-green algae (Myxophyceae) having pectin, cellulose and hemicellulose compounds such as xylan, arabinoxylan in their structure could act as a precursor to the production of cellulase, xylanase and pectinase enzymes in fungi. In this work, the effect of green algae extract ; Spirogyra sp. at 500 and 1000 mg/mL concentration on the rate of pectinase enzyme secretion and the relative expression of PgaA gene at 4 and 8 day after adding algae extract to culture medium was evaluated using qRT-PCR analysis. . The highest level of enzyme secretion was observed at 8th days after algae extract adding to culture medium at 1000 mg/mL concentration( 4.28 U/ml). Gene expression analysis of PgaA gene showed that the highest expression level was relted to 4th days time interval after adding alge extract at 1000 mg.mL (10/142). According to positive effect of spirogyra algae compounds on expression level of PgaA gene responsible to pectinase production and increase of enzyme secretion, it is concluded that microalgaes could be used as important sources of enzyme production from favorable fungi and could be useful in biotechnological industry