Effect Interaction of Endurance Training and Anabolic-Androgenic Steroids on Adipose Tissue’s Desnutrin, CD36, and Beta-HAD of Skeletal Muscle in Healthy Male Rats

It seems that endurance training and Stanozolol consumption can activate fat burning. The purpose of the present study was to investigate the effects of six weeks of endurance training and injection of stanozolol on CD36 and Beta- hydroxyl acyl coA dehydrogenases (B-HAD) levels of muscle tissue and levels of desnutrin in subcutaneous and visceral adipose tissue in male healthy rats.

In the present study, 40 male Wistar rats, aged 12 weeks old (initial body weight, 289±16g), were randomly divided into four groups; placebo (n=10), training (n=10), training+ stanozolol (n=10), and stanozolol (n=10). Training+ stanozolol and stanozolol groups received a weekly intramuscular injection (5mg/kg of body weight) of stanozolol, while placebo and training + placebo groups received Arashiz oil as placebo. Endurance training was performed on treadmill for 6 weeks, 5 sessions per week with the intensity of 70-75% VO2max. CD36, B-HAD, and Desnutrin levels were measured using ELISA method

After six weeks of exercise training and Stanozolol consumption, the protein content of CD36 in training+Stanozolol significantly increased compared to placebo group (P=0.0003), training+placebo group (P= 0.008), and Stanozolol group (P= 0.029). Protein content of B-HAD soleus muscle in training+Stanozolol significantly increased compared to placebo group (P=0.0002), training+placebo group (P= 0.006), and Stanozolol group (P= 0.018). Also, desnutrin of subcutaneous adipose tissue in training+Stanozolol significantly increased as compared to placebo (P=0.0005) and Stanozolol groups (P= 0.021). Desnutrin of visceral fat in training+Stanozolol significantly increased compared to placebo group (P=0.0007), training+placebo (P=0.039), and Stanozolol group (P= 0.004).

It seems that stanozolol treatment improves lipolytic effects of endurance training to significant extends.