Optimization in vitro micropropagation of peony (Paeonia mascula) ‎

This research was conducted in two stages to optimize in vitro propagation of Persian wild paeony. Stage 1: effect of BAP (0, 0.5, 1 and 2 mg.l-1), NAA (0, 0.1, 0.2 and 0.3 mg.l-1) and GA3 (0 and 0.5 mg.l-1) on apical bud ofPaeonia mascula and stage 2: effect of BAP (0, 0.5, 1 and 2 mg.l-1) and NAA (0, 0.25, 0.5 and 1 mg.l-1) were studied on embryonic callus induction on young leaves of Paonia mascula. At stage 1: the highest shoots number (5.38) was obtained on media containing 1 mg.l-1 BAP + 0.1 mg.l-1 NAA+ 0.5 mg.l-1 GA3. The results showed that increasing BAP level caused decreasing of shoot length. At stage 2: maximum of compact embryonic callus (17.93%) and somatic embryo number per explant (7.11) were obtained on 2 mg.l-1 BAP and 0.5mg.l-1 NAA. For callus proliferation, calli were maintained on MS medium containing 2 mg.l-1 BAP, 0.1 mg.l-1 NAA and 0.5 mg.l-1 GA3. Maximum rooting percentage (70.51%) and root number (0.0.02) were obtained with 0.25 mg.l-1 NAA.