study of comparative effects of harmine and ghrelin agonist on insulin and c-peptide secretion

The ability to secrete insulin from the islets of Langerhans on the one hand and suppress the factors that increase serum glucose concentrations on the other; It is very important in patients with metabolic disorders such as diabetes. The aim of this study was to compare the effects of harmine with ghrelin agonist (as a suppressor of insulin secretion) on insulin secretion and c-peptide in PANC-1 cell line and intracellular glucose concentration in HT1080 cell line.

To do this, cell lines were purchased from the Iranian Genetic Resources Center and after cell passage and preparation, were treated with doses of 0/01, 0/1, 1, 10 and 100 μg /ml harmin and ghrelin agonists with doses of 0/05, 0/5, 5, 50 and 500 μg / ml. While performing MTT test, insulin secretion and c-peptide were measured by ELISA.

The results showed that harmine dose-dependently increased insulin and c-peptide secretion as well as increased intracellular glucose and had the opposite effect of ghrelin agonist.

Harmine analogues could be a unique therapeutic promise for human diabetes and possibly serve as inhibitors of ghrelin agonists.