The Effect of Two-month Concurrent Training with and without Calorie Restriction on Sirtuin-1 Activity and Content in the Peripheral Blood Mononuclear Cells of Inactive Men

AbstractSirtuins have become prominent molecules in the aging process. Therefore, the present study was conducted to compare the effect of two months of concurrent training with and without calorie restriction on sirtuin-1 activity and content in the peripheral blood mononuclear cells (PBMCs) of inactive men. Therefore, thirty inactive men participated in three homogeneous groups: calorie restriction (CR: n=10), concurrent training (T: n=10), and concurrent training with calorie restriction (TCR: n=10). The calorie restriction was about -30 kcal•kg-1•week-1. The training consisted of five days a week (Resistance Training: two sessions / week-1, and High- Intensity Interval Training: three sessions / week-1). Sirtuin-1 content and activity in PBMCs were measured by ELISA and fluorometric assay, respectively. To measure oxidative stress, Plasma total antioxidant capacity (TAC) and serum malondialdehyde (MDA) levels were determined by FRAP and spectrophotometry, respectively. Data were analyzed using analysis of variance at a significance level ≤ 0.05. The results showed that Sirtuin-1 activity and TAC of all three groups were significantly increased after two-month interventions (p < 0.05). However, the changes in sirtuin-1 content and MDA levels were not significant in any groups (P> 0.05). Given the optimal changes in sirtuin-1 activity in PBMCs, TAC, and somebody composition components following two-months of concurrent training without calorie restriction, it can be concluded that concurrent training may be a more appropriate intervention for modulating the cell survival indicator in inactive men.