Effect of aqueous and ethanolic extracts of Carum copticum ( Ammi trachyspermum) and Stachys Lavandulifolia (Stachys lavandulifolia ) on Streptococcus iniae causative of streptococcusis

Streptococcusis is an infectious septicemic disease in fish .The frequent use of antibiotics in the aquaculture industry has led to the bio-distribution of these compounds in the edible tissues of fish and the emergence of drug-resistant bacterial pathogens in this industry. One of the strategies to tackle and prevent the emergence of antibiotic resistance is the use of herbal extracts and essential oils against pathogenic bacteria. The present study was carried out to use the plant extracts of Carumcopticum and Stachys lavandulifolia against Streptococcus iniae as zoonosis pathogen. Determination of minimum inhibitory concentration (MIC) and minimum inhibitory concentration (MBC) was used to determine the antibacterial strength of the extracts from the standard emission method in the disk. The minimum germination concentration of ethanolic and aqueous extracts of Carum copticum were 18.75 mg/ml and 75 mg/ml, respectively, and the minimum concentration of alcoholic and aqueous extracts of Stachys lavandulifolia were also, 18.85 mg/ml and a concentration of 75 mg/ml. As a result, ethanolic extracts of Carum copticum and Stachys lavandulifolia showed the highest antibacterial activity against Streptococcus iniae (P<0.05). It can be concluded that due to the proper effect of these extracts on Streptococcus iniae, it is necessary to evaluate the possibility of their use in the treatment and prevention of bacterial diseases resulted from this bacteria in fish