Effect of Cyclophosphamide on miRNA-15a expression in EBV infected transformed B cell

Despite advances in diagnosis and therapy of cancer, it is still the main cause of death worldwide. Cyclophosphamide is one of the major drugs for lymphoma treatment. However, the anti-lymphoma effects of cyclophosphamide on micro-RNA expression are still unclear, especially in B cell lymphomas. The aim of the present study was to evaluate the effect of cyclophosphamide on miRNA-15a genes expression in Epstein‐Barr‐Virus (EBV)-infected transformed B cell.

In this study, the effects of cyclophosphamide on CO 88BV59-1 LCL – an EBV infected transformed B cell was evaluated. The cells were treated with cyclophosphamide (0.05-50 µM), for 3 days. The cell viability and cytotoxicity were evaluated using MTT, and flow cytometry techniques, respectively. Also, miRNA-15a genes expression were determined by real-time PCR.

Cyclophosphamide significantly inhibited proliferation and induced cell death in the cell-line in a dose and time-dependent manner (P<0.05). There were no significant changes in the expression of miRNA-15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05).

The results showed that cytotoxic effects of cyclophosphamide on CO 88BV59-1 LCL is not related to miRNA-15a3p and miRNA-15a5p expressions.