Investigation of bioavailability of zinc oxide nanoparticles by Maytilaster lineatus and Dressina poly morpha bivalves in short term

In this study, the direct absorption of zinc oxide nanoparticles by M. linearatus and D. polymorpha was investigated over a short period of 96 hours. Sixty D. polymorpha legs with a range of 2.53±0.85 cm and sixty M. linearatus with a range of 2.7±0.5 cm was taken from the natural environment. The nanoparticles were spread using an ultrasonic device at 400 rpm. In order to phase together water reservoirs with nanoparticle solution by homogenizer device with 14000 rpm was used and Treatments were prepared at concentrations of 0.25, 25 and 50 ppm. The accumulation of nanoparticles in bivalve tissue mass with an ICP device and the distribution of nanoparticles in bivalve reservoirs were measured by DLS test. The results of ICP showed the highest accumulation of nanoparticles in bilayer tissue mass at the highest exposure concentration (p < 0.05). The lowest uptake was observed at the lowest exposure concentration (p < 0.05) compared to other treatments. D. polymorpha bivalve showed higher uptake ability than the bivalve M. lineatus during the exposure period. The DLS test results showed that the particles were in the range of 10-110 nm in size, confirming the non-segregation and homogeneity of the nanoparticles in the tanks. Both bivalve species are suggested as very good indicators for monitoring the effects of zinc oxide nanoparticles in the aquatic ecosystem.