Alloimmune lymphocytes proliferation in presence of Mixed Lymphocyte Culture and cyclosporine

Mixed Lymphocytes Culture (MLC), is a method studies the cell-cell interaction between subpopulations of lymphocytes and the production of compounds caused by this interaction. MLC as a source of cytokines can increase B lymphocytes proliferation. Repeated blood transfusions cause B lymphocytes alloimmunization. Cyclosporin A has inhibitory effect on immune system. In alloimmunization, stimulated B lymphocytes can produce antibodies against exposed foreign red blood cell antigens. Immortalization of B lymphocytes can lead to continuous production of monoclonal antibodies. Considering MLC as an important source of cytokines and the presence of B lymphocytes as a group of cells in  peripheral blood cells, the aim of this study was to produce MLC and evaluate its effect on proliferation of alloimmune peripheral blood cells in the presence or absence of cyclosporine A using microscopic evaluation and dye exclusion staining method.

In an experimental study, RhD antigen positive and negative lymphocytes were exposed together for MLC production. Peripheral blood lymphocytes resulted from two alloimmunized patients was treated with MLC and cyclosporine, and their proliferation was analyzed compared to the control group using microscopy and trypan blue staining method. The results were analyzed by t-test using prism software (p< 0.05).

MLC and cyclosporine increased proliferation of lymphocytes with mean value being 440000 (SD: 197989.8) vs 160000 (SD: 84852.8) of control group.

Conclusions  :

MLC and cyclosporine increase lymphocyte cells proliferation. By confirmation of additional experiments, MLC and cyclosporine may be used as effective agents in lymphocyte proliferation and antibody production of alloimmune B lymphocytes.