The Effect of 12 Weeks of High Fat Diet and Moderate Intensity Training on the KLF-15 / KLF-3 / Furin / CTRP-12 Pathway of Visceral Adipose Tissue in Male Rats

One of the factors that counteracts with risk factors of metabolic disorder is CTRP-12. Obesity plays an important role in the downregulation of CTRP-12 by positive regulation of furin and KLF-3 factors and negative regulation of KLF-15. Training affects adipocytes signaling. Therefore, the aim of the present study was to investigate the effect of 12 weeks of moderate intensity continuous training (MICT) on the KLF-15 / KLF-3 / furin / CTRP-12 of visceral adipose tissue in male obese rats. 15 male Wistar rats were divided into two groups: high fat diet (n=10) and standard diet (n=5). After 12 weeks, the high fat diet group was again divided into two groups: MICT and HFD. Training program was performed for 12 weeks, 5 sessions per week with intensity of 50-60% of maximum speed. The values of variables were measured by ELISA method. Data were analyzed using ANOVA and Prism 5 software. The results of this study showed that HFD significantly increased the amount of furin and KLF-3 in visceral adipose tissue (P≤0.05). The 12 weeks of MICT significantly decreased the levels of furin, KLF-3 and significantly increased KLF-15 compared with the HFD group (P≤0.05). Changes in CTRP-12 were not significant between groups (F = 1.032, P = 0.386). 12 weeks of MICT, apart from changes in CTRP-12, was effective in decreasing the risk of adipose tissue cellular factors (furin and KLF-3) and in increasing the beneficial factors of adipose tissue (KLF-15). Therefore, it is recommended that those exposed to obesity should get longer training sessions for better cellular adaptation.