Identification of Anura in the South East of Tehran Province by Histological Studies

The identification of Anura amphibians species in the southeastern cities of Tehran province was examined through histological studies. 4 main stations (i.e. Pakdasht, Varamin, Qarchak, Pishva) were selected in the southeast of Tehran province. Then, 24 samples specimens were preserved at 10% formalin solution and transferred to the laboratory for histological studies. Studies have shown that in one group of samples (Green Toad, Pseudepidalea (Bufo) viridis) there were toxin-producing glands. In this group, the 3-layer epithelium and below it the chromatophores were quite visible. Beneath them were large glands that produce toxins, followed by connective tissue and then striped muscles. In a group of amphibians (Marsh frog, Pelophylax (Rana) ridibunda) collected, a 4-layer epithelium was observed that divided itself into two subgroups: some with mucosal secretory glands and some without mucosal glands, but in the latter two groups, chromatophores, connective tissue, striped muscles and cartilage were seen. Another group of amphibians found that the epithelium was three-layered but had no venom-producing glands or no mucosal glands, but two features were seen in histological observations, such as some with cartilage and some without cartilage. The results of histological studies showed that in addition to morphological, morphological and genetic studies, histological studies can be used to identify of Anura. These differences indicate the possibility of polymorphism in the Marsh frog species.