Effects of Extrinsic Selenium Nanoparticles on the Qualitative Parameters of Frozen-Thawed Sperm of Broiler Breeder Roosters under Oxidative Stress Conditions

The objective of the present experiment was the study of selenium nanoparticle (NanoSe) in freezing extender on quality parameters of frozen-thawed sperm in broiler breeder roosters reared under physiological stress. Semen samples were collected from 12 birds Ross308, 28 week-age-old, which randomly allocated 2 groups included with physiologic stress (DEX group: using the injection of dexamethasone 0.4 mg/Kg body weight, three times, every other day for 1 week) or without dexamethasone injection (CON). Subsequently, after preliminary evaluation, samples collected from CON and DEX groups were divided into 4 experimental treatments for freezing using modified Beltsville extender either with or without NanoSe. Experimental treatments were included: 1) CON: control group, 2) DEX semen sample from dexamethasone group, 3) CONNSe: inclusion of NanoSe in CON semen sample, and 4) DEXNSe: inclusion of NanoSe in the DEX semen sample. Results show inclusion of Nano selenium in extender of CONNSe improved the Total motility (TM), Progressive Motility (PM), Average Path Velocity (VAP), Straight Line Velocity (VSL), Curvi Linear Velocity (VCL), Linearity (LIN), Straightness (STR), Lateral Head Displacement (ALH) and Beat Cross Frequency (BCF) in comparison with other experimental groups (P<0.05) Also, the Viability and Integrity of sperm Membrane, Glutathione Peroxidase (GPX) and Superoxide Dismutase (SOD) activities were higher in mentioned groups then other experimental groups (P<0.05) but there were no significant differences among groups in Malondialdehyde (MDA) and Total Antioxidant Capacity (TAC) (P>0.05). While the inclusion of NanoSe had no positive effects in DEXNSe versus DEX group (P>0.05). It seems the inclusion of NanoSe in extender improves the quality of frozen-thawed sperm, while cannot compensate for the negative effects of oxidative stress.