lnter-specific hybridization between Lolium species

A number of populations of three Loriurn species including L_ mu.worum, L. rigidum and L' perenne, were investigated for cytogenetic characteristics, such as ploidy level, in order to be used in breeding projects of the species. Seeds were germinated and fresh grown root tips were treated with alpha bromonaphtalin, Farmer solution, 1N HCI and hematoxilin as pretreatment, fixative, hydrolize agent and chromosome staining dye, respectively. Mitotic cells were captured at metaphase stage and karyotypic characteristics such as ploidy lever were investigated' A number of populations were tetraploid ancl the rest cliploid. The phenomenon focused in this article was mixoploicly and aneuploicly in the studied populations. Two populations of L. rigidum and L. prenne trisomic phenomenon was observed. Also mixoploidy was observe d in L. igidum and, L. murffiorum, by two levels of polyploidy.