Evaluation of microwave irradiation and toxin adsorbents on Diazinon residues in ‎white grape pomace and its effect on some ruminal parameters in vitro condition

The aim of this research was to determine effects of microwave irradiation and different toxin adsorbents on the amount of Diazinon residues in white grape pomace and effectiveness of these processes on some rumen parameters and gas production in vitro condition. This study, were investigated in a completely randomized design with 5 treatments and 5 replications. Experimental treatments included: 1- White grape pomace without processed or control group 2- Grape pomace processed with microwave irradiation 3- Grape pomace processed with Mycofix-Plus toxin adsorbent 4- Grape pomace processed with Bio-Tox toxin adsorbent 5- Grape pomace processed with Bio-Acid toxin adsorbent. The results of this study showed a significant increase in the amount of dry matter, neutral detergent fiber and acid detergent fiber in the microwave treatment (P˂0.05). Different processes reduced the amount of Diazinon in the white grape pomace (P˂0.05), so the highest amount of Diazinon in control treatment (3.86 mg/kg) and the lowest amount of toxin was observed in treatment group treated with Bio-Tox supplement (0.57 mg/kg). Different processes increased in vitro gas production, total VFA, propionic acid concentration, dry matter digestibility and Methane (P˂0.05). Different processes reduced acetate concentration and did not have a significant effect on the Rumen protozoa population (P>0.05). Adding different adsorbents increased butyrate and isobutyrate concentration (p<0.05). As a conclusion, microwave irradiation and different toxin adsorbents reduced the amount of Diazinon in the white grape pomace and improved some ruminal parameters in vitro condition.