The effect of morphometery, viscosity and liquefaction on frozen sperm quality of Ghezel ram

The present study was performed to evaluate the effect of morphometry, viscosity and liquefaction of semen on frozen sperm quality. Semen was collected once a week for 3 weeks from 5 Ghezel rams. Total and progressive motility, viability, abnormal sperm, concentration, viscosity, and liquefaction were studied in fresh sperm. After dilution the suitable samples, straws (0.25 ml) were filled and after cooling and reaching to 5 ° C, are placed in 4 cm above liquid nitrogen for 8 minutes and then, ultimately were immersed in liquid nitrogen. Samples were thawed on days 0, 20, 40, and 60 of the study to evaluate the characteristics of total motility, progressive motility, viability, plasma membrane health, acrosome integrity, and abnormal sperm percentage. The results of this study showed a negative and significant correlation between drop length and plasma membrane health (p <0.05). Also, it was shown that there was a positive and significant correlation between semen string length and total motility (p <0.01). There was a significant negative correlation between liquefaction and total motility, progressive motility and acrosome integrity (p <0.01). There was a significant negative correlation between tail length and total sperm length with plasma membrane health (p <0.01). As well as, there was no correlation between sperm head length and width with relevant parameters (p >0.05). Whatever, the liquefaction time, sperm tail length and total length of sperm was smaller, the qualitative traits of sperm will be better preserved in the freezing process.