The Effect of a NSAID Drug on the Expression of Inflammatory Genes in Prostate Cancer Cells

Inflammation is a physiological process begining with tissue damage in the presence of various infectious agents, and men with symptoms of chronic inflammation in their noncancerous prostate tissue are about twice as likely as other men to develop advanced prostate cancer. Considering the importance of inflammatory factors in the invasion and metastasis of prostate cancer cells, we decided to evaluate the effect of the anti-inflammatory drug M2000 on two inflammatory genes involved in the invasion of cancer cells. PC3 cells were first cultured using RPMI medium with 10% FBS and 1% PenStrep. The cells were then treated with M2000 at concentrations of 25 and 50 μg/ml and after 24 hours the expression of il-8 and NF-κB were examined using real-time RT-PCR. NF-κB was assessed using real-time RT-PCR. In this study, NF-κB gene expression decreased in all treatments in RT-PCR. While the il-8 decreases in expression at a concentration of 50 μg/ml. The results of the present study showed that M2000 can reduce the expression of il-8 and NF-κB genes involved in tumorigenesis and invasion of prostate cancer cells, which could pave the way for future studies to better control prostate cancer.