Association of Polymorphisms of F2 (rs3136520) and PAI-1(rs6090) Genes with Recurrent Pregnancy Loss in Iranian Azeri Women

The mutation of F2 and PAI-1 genes can cause thrombophilia in women. The present study aimed to assess the relationship of recurrent pregnancy loss (RPL) with rs3136520 in F2 and rs6090 in PAI-1.

This case-control study was conducted on 120 women with idiopathic RPL as case group and 120 healthy women without any history of infertility or abortion with at least one healthy child as the control group. These subjects were referred to a private genetic lab by a gynecologist. Both polymorphisms were studied using the amplification-refractory mutation system-polymerase chain reaction approach, and the obtained data were analyzed in SPSS software (version 26) using the chi-squared test (P˂0.05).

In the multiplicative model, there was a significant association between rs6090 and RPL (OR=0.222; 95%CI=0.082-0.599); nonetheless,
no relationship was detected between rs3136520 and RPL (OR=1, 95%CI=0.488-2.049). Moreover, according to additive model, there was no association between rs3136520 and RPL; however, there was a significant relationship between AG genotype in rs6090 and RPL (OR=0.245; 95% CI=0.066-0.901).

As evidenced by the obtained results, there was a protective association between rs6090 and RPL; nonetheless, there was no relationship between rs3136520 and RPL.