Evaluation of Changes in Lung Tissue Antioxidant Enzymes after Endurance Training with The Berberine Chloride Usage in Diabetic Wistar Rats

Aerobic training and berberine chloride both have antioxidant characteristics, therefore the present study aimed to evaluate the changes in lung tissue antioxidant enzymes after endurance training with the use of berberine chloride in diabetic vestibular rats. For this purpose, 40 adult male Wistar rats weighing approximately 200-250 g were randomly divided into 5 groups including healthy control ,diabetic control group (with STZ injection), diabetic experimental group+physical Exercise, diabetic experimental group + berberine Intake (50 mg/kg), diabetic experimental group+physical exercise+berberine Intake. 24 hours after the last exercise session, the animals were anesthetized with an intraperitoneal injection of Ketamine and lung tissue was harvested to measure the antioxidant enzyme activity of superoxide dismutase, catalase, and glutathione peroxidase. Paired t-test and ANOVA were used to analyze the data using SPSS software (version 22) with significant level α=0.05. The results showed that compare to control group, the level of CAT, SOD and GPX activity was significantly higher in all of the experimental groups (P=0.001). In comparison to the diabetic + training group and the diabetic + berbine group, the effects of intervention was significantly higher in the diabetic+training+berberine group. The final conclusion indicated that endurance training with berberin chloride consumption can cause a significant increase in the anti-oxidant enzyme activity in the lung tissue of diabetic rats.