Mixotroph culture of (Chlorella vulgaris) microalgae under nitrogen stress to produce an extract edible oil

For several years, microalgae have been known to promise sustainable and cost-effectiveFor several years, microalgae have been known to promise sustainable and cost-effective production of food, fuel and chemicals. Many algal species produce lipids as triacylglycerols with fatty acid compounds similar to vegetable oils (TAGs). Therefore, microalgae are a good choice for the sustainable production of natural edible oils. In this study, the effect of mixotroph culture and nitrogen shock on dry weight, fat content and profile of fatty acids of Chlorella vulgaris were investigated. In this study, BBM culture medium used at temperature 25°C to grow microalgae. Cell count indices, nitrate, pH, dry weight, fat weight, fat percentage and profile of fatty acids were measured. The results showed that mixotroph culture and nitrogen shock are significant on biomass and fat content (p <0.05). Based on the results, it was determined that for dry weight, the glucose mixotroph with 100% nitrate in BBM medium 916.66 ± 35.11 mg/l, for the amount of fat in mixotrophic culture, glucose with 25% nitrate in BBM culture medium 49.61 ± 0.36 mg/l and for linolenic acid (omega 3) autotrophic culture with 100% nitrate 19.72 ± 0.1% had the highest production values. In conclusion, nitrogen shock reduces cell biomass and increases microalgae oil, as well as decreases omega-3 fatty acids.