The study of hydroalcoholic extract of Thymus vulgaris on visceral pain with a writhing test in mice

Visceral pain is an unpleasant feeling that Using synthetic drugs for treatment has some side effects, such as psychological dependence and blood dyscrasia and for decreasing these side effects, administration of herbal medicine can be an appropriate approach. Based on existing information in traditional medicine and some recent scientific surveys, Thymus vulgaris plant is one type of herbs that has different therapeutic effects of which it is possible to point to antibacterial and antifungal effects and its application in some respiratory disorders The aim of this study is evaluation of the palliative effect of Thymus vulgaris on visceral pain. This experimental study was done on 40 NMRI male mice. (36±4g). Mice were divided randomly into control groups, positive group (flunixin), treatment 1, treatment 2, treatment 3. Control groups and positive group, normal saline and flunixin (2mg/kg) and also treatment groups respectively 150, 300, 450 mg/kg of Thymus vulgaris received intraperitoneally. 15minute after each injection, for visceral pain induction, mice were injected by acetic acid 0.6% (10ml/kg) and during  30 minutes, the analgesic effect was recorded by counting the number of Writing. Data were analyzed by SPSS statistical software, One-Way ANOVA and Tukey test with a significant level of P< 0.05. The extracts of Thymus with 150, 300, 450 mg/kg doses and flunixin with 2 mg/kg dose induced a significant reduction in pain response. (P<0.05). This study confirms the palliative properties of Thymus Vulgaris, but further clinical studies are necessary to find out a suitable place for that among the other visceral Analgesics.