Morphology of erytrocyte cells of Triploid hybrid, rainbow trout and Caspian salmon

The aim of this study was to investigate the effect of mixing on the morphology of erytrocyte cells in triploid hybrid fish species compared to parents, as well as comparing some of the abnormalities of the thrombolytic kidney, Rainbow trout and Caspian salmon. In order to induce the triploid, thermal shock at 28 ° C and the time of shock initiation were performed 10 minutes after fertilization for 10 minutes. The results of the measurement of erytrocyte cells showed that in the triploid hybrid mixture compared to the parent, the large axis of the cell had a significant increase in the small and large axis of the nucleus. However, the small axis of the erytrocyte cells of the triploid hybrid showed a significant decrease compared to the control groups. The results of comparing the amount of cells and nuclei in the triploid hybrid combination compared to the control groups showed that the cell, nucleus and nucleus volume in the triploid hybrid increased significantly compared to the control groups   (P <0.05). But the area of the cell in the mixture was not significantly different in comparison with the control groups (P> 0.05). The major axis of the nucleus or cell increased more relative to its corresponding width. erytrocyte cells in the Triploid hybrid showed more abnormalities in comparison to the control groups, mainly due to the presence of cells with divided nuclei, the presence of red blood cells without nuclei and abundance higher than the type of immature erytrocyte cells in the blood stream. Cell death, cell membrane destruction of large erytrocyte cells through the passage of blood capillaries or early release of immature erytrocyte cells, is the main cause of these abnormalities. The results showed that mixing causes these abnormalities in the blood and thus leads to a decrease in the biological efficiency of the triploid hybrid in comparison with the diploid groups.