Compared the Effect of Red and Infrared Low-Level Lasers on Folliculogenesis Cycle with the Effect of Clomiphene Drug in Rat’s Ovarian Tissue

Folliculogenesis is a cycle that any disruption in this cycle leads to disease. Treatment include Surgery, drugs and low-power lasers have been used as complementary. This study investigated photo-bio-stimulation effect of lasers (power<250 mW)) on folliculogenesis cycle in rats’ ovarian tissue in two wavelength 630 and 810 nm autonomously.

Twenty-eight female Wistar rats in weights between 150-250g at the age of 9 weeks divided into four groups: control (CT), Drug(D), Red Laser (RL) and Near Infrared Laser (NIRL). For this, two diode lasers with an output 33±2 mw/cm2 for RL and 212±2 mw/cm2 for NIRL have been used. In the first 2 days of estrous cycle the laser groups were irradiated (5j/cm2 dose). The rats in D group received clomiphene (1μg/Kg). After the experiments, the rats were anesthetized and sacrificed and their ovarian tissues were removed.

The number of different follicles and the levels of effective hormones were evaluated. The results showed a change in follicles and hormones compared with D and CT groups. This change was significant in the NIRL group compared with other groups, while it was not significant in the RL group. Theas results in D group was less than laser groups and was significant only compared with CT group.

Low-power laser therapy at 810 nm can increase the activity of ovarian tissue in the production of follicles independently. Therefore, this study was able to introduce 810 nm laser as a new independent treatment for ovarian hypoglycemia that can be used in future studies.