Extraction and Determination of Diazinon Poison by NiZnS-C Nanoparticles by Ultrasound Microextraction and Experimental Design from Environmental Waters

Organophosphates are the basis of many pesticides and chemical warfare agents. Organophosphorus pesticides (OPPs) are a class of chemicals that generally act as cholinesterase inhibitors and have been widely used in agriculture due to their high efficiency as insecticides. The main purpose of this study is to present a new method for extracting diazinon toxin from ambient water.

In this research NiZnS nonmaterial supported on the activated carbon synthesized and characterized with various method including XRD, FT-IR and TEM. Then these nanomaterials with 45 nm average particle size was applied for extraction of diazinon pesticide based on dispersive nano solid material-ultrasound assisted microextraction (DNSUAME) from environmental water samples prior to HPLC-UV. The optimum effective variables on the extraction yields were investigated by central composite design.

The optimized DNSUAME combined with HPLC-UV allowed quantification of trace levels of diazinon in the water samples. The advantages of the proposed method based on the new nanomaterial include simple, and inexpensive synthesis method; rapid and convenient extraction operation, feasibility for large-volume samples, high sensitivity, and precision and accuracy in preconcentration and determination of diazinon. 

At optimum conditions values of variables set as 15.5 mg of NiZnS-AC, 5.5 min vortex and 5.5 min sonication time. At optimum conditions method has linear response over 0.001-10 µgmL-1 with detection limit 0.0005 µgmL-1 with relative standard deviations (RSDs) less than 6 % (n=6).