Comparative study of impact of docetaxel on the cytoskeleton of mouse oocytes after vitrification with two different cryopreservation solutions

Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Inroduction & Objective

The aim of the present study was to investigate the effect of docetaxel on the survival rate and in vitro fertilization of oocytes after vitrification by two cryopreservation solution.

Materials and Methods

For this NMRI mice (8-10 weeks old) were superovulated by injecting PMSG and HCG. Oocytes are surrounded by cumulus and corona cells and must be denuded by 0.1% hyaluronidase enzyme. The oocytes were then divided into 8 experimental groups including control, docetaxel, docetaxel + vitrification 1 solution; docetaxel + vitrification1; vitrification1; docetaxel + vitrification 2 solution; docetaxel + vitrification2; vitrification2. Mature oocytes were vitrified in ethylene glycol and dimethyl sulfoxide solutions at 15% concentration and 0.5 M sucrose in cryopreservation solution1 and ethylene glycol and glycerol at 7.5 concentration and 0.5 M sucrose in cryopreservation solution2. After thawing, their survival and fertilization rates were assessed up to the two-cell stage. Staining of the microtubules in the oocytes was performed with alpha-tubulin antibody.

Results

The results showed a significant difference in survive and fertilization rates compared to the control group (P<0.05). The rate of survival and formation of 2-cell embryos in the first cryopreservation group decreased compared to the second cryopreservation group but the two groups were not statistically significant. The results showed that survival and fertilization rates in pre-incubated groups with docetaxel were higher than non-incubated groups.

Conclusion

Docetaxel could improve reproductive techniques by reducing the damage to the oocyte cytoskeleton.

Language:
Persian
Published:
Journal of Plasma and Biomarkers, Volume:15 Issue: 2, 2022
Pages:
123 to 135
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