The effect of 8 weeks caloric restriction on PLIN5 and ATGL visceral adipose tissue and insulin resistance in type 2 diabetic male rats

There is a close link between eating fatty foods, the prevalence of obesity and type 2 diabetes. This study aimed to evaluation the effect of 8 weeks caloric restriction on Perilipins (PLIN5) and ATGL visceral adipose tissue and insulin resistance in male diabetic rats.

In this experimental study, 24 male rats were divided into healthy control, diabetic control and diabetic group with caloric restriction. In the caloric restriction group, %40 of the calorie intake was restricted and other groups received the standard food they needed freely for 8 week. The relative expression of prilipine 5 and ATGL protein by Western blotting and insulin resistance were also evaluated using Homa index. Data were analyzed by one-way ANOVA and Tukey's post hoc test.

A significant difference was showed between the means of the three groups in comparing the expression of PLIN5 and ATGL proteins. Post hoc test showed a significant increase in PLIN5 and ATGL in the caloric restriction group compared to the healthy and diabetic control group (P=0.001). There was also a significant decrease in insulin and glucose levels in the caloric restriction group compared to the diabetic control group (p = 0.001).

Calorie restriction may prevent insulin resistance by acting on the overlying proteins and thus reducing fat accumulation.