Appraising Antioxidant and Antibacterial Activities of Zinc Oxide Nanoparticles Synthesized Biologically by Iraqi Propolis

The biosynthesis of nanoparticles using natural products has recently attracted a lot of interest. The present study has investigated anti-biological activity of Zinc oxide nanoparticles (ZnONPs) synthesized biological by Iraqi propolis extract. ZnO NPs are well-known nanoparticles that are cost-effective, non-toxic and biocompatible. The antioxidant of ZnONPs was determined by using the free radical 2,2–diphenyl-1-picrylhydrazylhydrate (DPPH) assay, with different concentrations of the NPs. The results exhibited that with scavenging activity, the highest scavenging at 400µl/ml reached 74.70%, compared with ascorbic acid, which was used as standard at the same concentration reaching 77.56% (P≤0.05). The evolution of IC50 was 164.31 to ZnONPs compared with ascorbic acid IC50 =111.42. The antibacterial activity of the synthesized ZnONps was determined by using a disk diffusion assay. The results indicated effective antibacterial activity both in Gram-negative and Gram-positive bacteria in different concentration.