The Interactive Effect of Running on Different Slopes with the Use of Royal Jelly on the Concentration of Some Antioxidants in an Animal Model of Trimethyltin-treated Alzheimer’s

Although the role of exercise and royal jelly in the health of Alzheimer’s patients has been reported, the interaction effect of these two variables on superoxide dismutase (SOD) and glutathione peroxidase (GPx) is still not well understood. Therefore, the present study was aimed at investigating the interactive effect of positive slope running (PSR) and negative slope running (NSR) with royal jelly on SOD and GPx concentrations in a trimethyltin (TMT) Alzheimer’s animal model. In this experimental trial, 49 rats in the age range of 8-10 weeks, weighing 190-220 g, Alzheimer’s with 8 mg/kg TMT were assigned into seven groups including group one: TMT, group two: sham, group three: Royal jelly, group four: positive slope, group five: negative slope, group six: royal jelly + positive slope and group seven: Royal jelly + negative slope. Moreover, to investigate the effect of Alzheimer’s induction on the research variables, seven healthy rats were included in the healthy control group following the positive slope and negative slope exercises for eight weeks, three sessions per week and each session ran for 60 minutes on a positive 15 and negative 15 slope on the treadmill. Besides, 100 mg/kg of royal jelly was peripherally injected into rats daily. One-way analysis of variance (ANOVA) with Tukey post hoc test and two-way ANOVA with Bonferroni post hoc test in SPSS 22 software were used to analyze the data (p ≤ 0.05). Negative slope increased SOD and GPx in hippocampal tissue of AD rats. PSR and royal jelly increased GPx. Positive slope and royal jelly and negative slope and royal jelly groups had a significant effect on increasing SOD and GPx. Positive and negative slopes and royal jelly seem to improve antioxidant defense; furthermore, the interaction of positive slope and royal jelly and the interaction of negative slope and royal jelly are more desirable than the effect of each alone.