Fingerprinting of Aegilops species using genes-targeted drived and conserved regiens CoRAP markers

Aegilops range grasses (Poaceae family) as one of the most important ancestors of wheat is a rich source of stresses tolerance genes. For this, the fingerprinting of genetic relationships in accessions belonging to eight species of Aegilops was studied using targeted genes-related CoRAP molecular markers. In designing the fixed primers, six genes CAT, MnSoD, SoS1, miR398, miR160b and miR169gR responsible for abiotic stresses tolerance were used. The lowest and highest polymorphic information contents were related to CoRAP2 and CoRAP10 primers with 0.92 and 0.96, respectively. The marker index varied from 6.89 in CoRAP7 primer to 13.49 in CoRAP9 primer. Low gene flow (Nm) and high differentiation (Gst) was observed between species. Also, the Fst index equal to 0.45 showed that the studied populations were completely separated. Due to the high values of the effective allele number and Nei genetic diversity, two species Ae. cylandrica and Ae. caudata showed high intra-specific diversity. The greatest similarity was observed between two species Ae. truncialis and Ae. umbelulata as well as Ae. neglecta and Ae. cylanrica. Cluster analysis appropriately divided species into distinct groups, and principal coordinate analysis confirmed the results of cluster analysis. Using structure analysis of the population, the mode of gene flow and genetic intermixture among species were in accordance with the grouping results of cluster analysis and PCoA biplot.