Isolation and Molecular Identification of Antibacterial-Producing Actinomycetes against Pathogenic Pathogens from Industrial Effluent Sediments of the Maragheh Glass Factory: A Laboratory Study

Due to the increasing of antibiotic resistance, the use of new antibiotic sources, especially actinomycetes, as new sources of antibiotics have been considered. The aim of this study was to isolate and identify actinomycetes with the ability to produce antibacterial metabolites from industrial effluent sediments and study their antimicrobial effect.

In this laboratory study carried out in 2019, actinomycetes were collected and isolated from the sediments of industrial wastewater of Maragheh Kaveh Soda Glass Factory. Actinomycete isolates were screened by Starch casein agar medium based on the formation of inhibitory zones. Also, anti-biogram test was performed by disk diffusion method on 20 patients of Tabriz medical centers. The results were analyzed by SPSS software version 23.

Based on E-Test approach, all isolates were sensitive to Vancomycin antibiotic and had lower MIC (Minimal inhibitory concentration) value of less than 2 μg/ml. The highest rate of antibiotic resistance to erythromycin and amoxicillin was observed in the included isolates. The results indicated that among 100 actinomycete isolates, five of them had the highest ability to inhibit some of bacteria. S2 (16.46±1.43) and S39 (18.25±1.44) strains demonstrated better activity against methicillin-resistant Staphylococcus aureus (MRSA) compared to vancomycin antibiotic (14.14±1.23).

The results showed that industrial wastewaters of factories are containing active actinomycetes that could be used as new antibacterial metabolites.