Isolation, identification and evaluation of enzymes of a new psychrophilic bacterium from the intestine of Crustacean Gammarus sp collected from cold springs of Saman (Chahrmahal va Bakhtiari)

Psychrophilic bacteria are useful in biotechnology due to their ability to produce different enzymes that capable of catalyzing biochemical reactions at low temperatures. Therefore, many studies have been done on these bacteria in recent years. The aim of this study was to isolate and identify the bacteria producing cold-adapted enzymes from the intestine of the new species of crustacean Gammarus species living in cold water spring of Saman in Chaharmahal va Bakhtiari province, Iran. The Gammarus samples were collected from cold spring of Saman. After transfer to the laboratory, bacteria were collected from the intestines in sterile condition. Then after, bacteria were cultured on the LB media and incubated at 5˚C temperature. Grown colonies were studied based on morphological characteristics, biochemical tests, and finally molecular identification was performed based on 16SrRNA sequence determination. A native strain of psychrophilic bacteria was isolated and identified, deposited with GenBank accession number MK961220. More experiments show that the new strain is capable of producing amylase, xylose isomerase, catalase, oxidase, and serine protease and laccase enzymes. The maximum activity and stability of most of the investigated enzymes were recorded at 5˚C. This study was conducted for the first time in the country to investigate the potential and ability of extracellular enzymes produce with biotechnological potential by the new psychrophilic strain. According to research, this strain can be used as a suitable candidate for the application of Cold adapted enzymes in various industries.