The Effects of Azathioprine on Kidny Tissue in Dibetic Rats

Diabetes is a metabolic disease that results in major damage to the kidney. Azathioprine is widely used in the treatment of other autoimmune diseases, cancer, and organ transplantation. In this study, the effect of azathioprine on renal tissue and serum markers of insulin resistance in rats were studied. 56 adult male wistar mouse with 80 to 90 days old and weighing approximately 200±20g were diveded in 8 groups as follow: Control group (without treatment), sham (140cc froctose 10% as a daily feeding) and 1 to 4 experimental groups that in addition of 140cc froctose 10% as a daily feeding, were injected [3.75, 7.5, 15 and 21] mg/kg/bw azathioprine both in single dose and interaperitoneal at 96th day. 24 hours after both kidneys were seprated, fixed in formalin (10%) and placed in tissue processor. Prepared serial sections were studied with LM (10x & 40x). Obtain data analyzed by using SPSS (17) and ANOVA and groups were compared in significance level (P< 0.05) with Tukey's test. Significant decrease in the diameter of the renal cortex and medulla in control, diabetic and non-diabetic treatment groups and a significant increase in proximal and distal convouluted, henle's loop and glomerules compared with the control group. Collecting tubes  difference was not significant in all groups compared to controls. According to above results, Azathioprine creating synergistic effects with diabete. Therefore, diabetics are taking limits.