Evaluation of total phenol and flavonoids, antioxidant potential and antimicrobial activity of sage extract on some Gram-positive and Gram-negative bacteria “in vitro”
Diseases caused by the use of chemical preservatives have increased the tendency to use natural antimicrobial and antioxidant compounds. In this study, the herbal extract of Salvia rhytidea Benth was extracted using ethanol solvent. The content of total phenols and flavonoids in the ethanolic extract were found to be 36.14 mg GAE/g and 17.13 mg QE/g, respectively, indicating the high antioxidant properties of the extract. The antioxidant activity of the extract based on IC50 in DPPH free radical scavenging and ABTS free radical scavenging was 75.32 and 66.60 mg/ml, respectively. Evaluation of antimicrobial properties of ethanolic extract of S. rhytidea by disk diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration showed that although the extract was more effective against Gram-positive bacteria than Gram-positive bacteria, the plant can be used as a natural preservative in various industries.
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