Detection of tms2 Gene Causing Crown Gall in Two Commercially Cut Flower Roses ("Pearl" And "Angelina") by a Nano Biosensor

In this study, the tms2 gene, the cause of crown gall, is detected in two Rosa hybrida L. (“Pearl” and “Angelina”) cultivars using electrochemical methods. In this study, stabilization, and hybridization of single-strand DNA (ssDNA) related to tms2 gene, Agrobacterium tumefaciens is investigated by electrochemical impedance spectroscopy. For this purpose, the pencil graphite electrode is modified with nanomaterials including reduced graphene oxide and gold nanoparticles. The biosensor could be used to detect infected samples of “Angelina” and “Pearl” roses. DNA/DNA hybridization is performed by placing an electrode modified with ssDNA at a concentration of two (µmol/L) target DNA. Results show proper stabilization and hybridization of DNA at the electrode surface. The designed biosensor has good selectivity. DNA-based nanosensor has advantages such as low cost, simplicity, and scalability and can be the basis for the development of genomic detection tools.