Identification of renal cell carcinoma specific genes and in vitro confirmation of their expression in ACHN cell line in the presence of Staphylococcus aureus toxin SHE

Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults. The aim of the present study was to obtain specific genes in RCC, based on bioinformatics studies and to evaluate the expression change of candidate genes in ACHN cells after transfection of the seh gene of Staphylococcus aureus.

The expression correlations of genes involved in RCC were investigated in the TCGA database, and networks of genes was constructed. In vivo studies were performed in the ACHN cell model, in which the staphylococcal seh gene was cloned into the pcDNA3.1 (+) vector and transfected into ACHN cells. After confirming the correct function of the recombinant vector in these cells and inducing apoptosis, the expression of candidate genes was evaluated.

The results showed the successful identification of the expression correlations of genes involved in apoptosis and lncRNA-mRNA interaction network in RCC. Induction of apoptosis under the influence of toxin expression was confirmed by flow cytometry. Expression of SOX9, SBF2/AS1, MAF and c-Maf genes in cells ordered by recombinant vector was significantly reduced compared to control cells. Also, increased expression of LINC00671 and LINC02499 genes was observed in cells transfected with pcDNA3.1 (+)- seh vector compared to the control group (p <0.05) and This change in expression confirmed the accurate identification of genes involved in RCC cell apoptosis.

The gene networks identified in this study can provide a new perspective for research aimed at identifying new molecular pathways and treating RCC.