Investigating the Antimicrobial Effect of Plant Essential Oils on Standard H37RV and Micobacterium Tuberculosis Clinical Isolates

Myrtus Communis is a shrub and evergreen plant that grows in the plains of Asian countries including Iran and Mediterranean countries. Since ancient times, the essential oil of this plant has been used as a disinfectant. In addition to its antiseptic effect, it is used as a stomach booster and also to cure respiratory and urinary tract diseases. The aim of this study was to determine the antimicrobial effect of the plant essential oil on the H37Rv standard strain and clinical isolates of Mycobacterium tuberculosis.

The plant essential oil was extracted using a Clevenger Apparatus, then using the Kirby-Bayer standard disk diffusion method, the antimicrobial property of the plant essential oil was determined by determining the minimum inhibitory concentration (MIC) against 4 different strains of Mycobacterium tuberculosis compared to the standard strain H37Rv in Four concentrations were evaluated.

In this study, it was shown that there was a significant difference between the different concentrations determined for microbe cultivation, in the plant essential oil, the minimum inhibitory concentration(MIC) against two bacterial strains was equal to (7.5 mg/ml). It is known that the minimum bactericidal concentration (MBC) of two strains was equal to (15 mg/ml). The highest inhibitory concentration was equal to (15 mg/ml) and one strain was inhibited at this concentration and the lowest inhibitory concentration was The concentration was equal to (3.75 mg/ml). Also, MIC and the MBC against the standard strain were (1.87 and 3.75 mg/ml) respectively. This shows the significant effect of the antimicrobial properties of the essential plant in reducing the growth of bacteria.

The results of this study indicate the positive effect of the essential oil of the case in the treatment of tuberculosis. Therefore, the compounds in the essential oil of this plant can be used to develop and produce new Tuberculosis drugs.