The Evaluation of Anxiolytic Effect of Hydroalcoholic Portulaca Oleracea Extract on Rats during Bisphenol A Toxicity

Bisphenol A is a potentially endocrine-disrupting chemical agent which is widely used in plastic industries and its wrecking effects on various body organs have been proved. Human is more exposed to BPA because it can be released from polymeric materials and enter water and food sources. Purslane is a plant with different kinds of phytochemical compounds and antioxidant and anti-inflammatory effects could modify and improve the toxic effects of BPA. Forty nine rats were randomly divided into 7 groups: control group, experiment group (receiving 500 µg of corn oil / kg BW), toxin-receiving group (500 µg of bisphenol A/kg BW), positive control (100 mg of hydroalcoholic extract of portulaca oleracea/kg BW), 3 treatment groups receiving purslane hydroalcoholic extract (500 μg of bisphenol A + 50, 100, 150 mg of hydroalcoholic extract of purslane hydroalcoholic extract/ kg BW, respectively). All injections are done by i.p rout in 14 consecutive days. Anxiety test is performed by elevated plus maze apparatus in the last day. Data was analyzed in SPSS statistical software by one-way ANOVA with Tukey posttest. The findings of the study indicated a significant increase (p ≤ 0.05) in the amount of Open Arm Time (OAT) and Open Arm Entries (OAE) as anxiety parameters in purslane treated groups compared with control group. The results indicate the dose-dependent effect of hydroalcoholic extract of purslane on the reduction of anxiety state. Also analyzed data showed that purslane can improve BPA toxic effects.